Coding

Part:BBa_K1807014:Design

Designed by: Ivan Gyulev   Group: iGEM15_York   (2015-09-17)


PstSCAB Operon from Sinorhizobium meliloti 2011


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 2489
    Illegal PstI site found at 2316
    Illegal PstI site found at 3963
    Illegal PstI site found at 4385
    Illegal PstI site found at 4424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 2489
    Illegal PstI site found at 2316
    Illegal PstI site found at 3963
    Illegal PstI site found at 4385
    Illegal PstI site found at 4424
    Illegal NotI site found at 2955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 2489
    Illegal BglII site found at 3791
    Illegal XhoI site found at 3346
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 2489
    Illegal PstI site found at 2316
    Illegal PstI site found at 3963
    Illegal PstI site found at 4385
    Illegal PstI site found at 4424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 205
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 2489
    Illegal PstI site found at 2316
    Illegal PstI site found at 3963
    Illegal PstI site found at 4385
    Illegal PstI site found at 4424
    Illegal NgoMIV site found at 88
    Illegal NgoMIV site found at 193
    Illegal NgoMIV site found at 295
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 1184
    Illegal NgoMIV site found at 1285
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 892
    Illegal BsaI site found at 2117
    Illegal BsaI site found at 2637
    Illegal BsaI site found at 2985
    Illegal BsaI site found at 3595
    Illegal SapI site found at 1289
    Illegal SapI site found at 1954
    Illegal SapI site found at 2536
    Illegal SapI site found at 4057
    Illegal SapI.rc site found at 3198


Design Notes

-


Source

Sinorhizobium meliloti 2011

References